An improved procedure of agarose gel isoelectric focusing.

Two-dimensional gel electrophoresis, isoelectric focusing and agarose gel electrophoresis in the diagnosis of multiple sclerosis.

Two dimensional gel electrophoresis (2-DE), isoelectric focusing (IEF) and agarose gel electrophoresis (AGE) were used to examine cerebrospinal fluid (CSF) and sera from 22 patients with confirmed multiple sclerosis, 11 patients with probable multiple sclerosis and 20 control patients with non-inflammatory neurological diseases of the central nervous system (CNS). All of the 22 patients with co...

Isoelectric focusing of proteins in rewetted ultrathin polyacrylamide gel layers.

Ultrathin layers of polyacrylamide gel bound to glass can be washed, air-dried, and stored for at least 1 year before rewetting in ampholyte solutions for isoelectric focusing. Short-term drying affects neither fluorescent banding of the ampholytes (not evident in conventional gels) nor resolution of complex protein mixtures while prolonged storage seems to have no deleterious effects. Layers a...

Two−dimensional separation of human plasma proteins using agarose gel isoelectric focusing followed by SDS capillary electrophoresis

Two−dimensional polyacryiamide gel electrophoresis (2−D PAGE) has been widely used for protein separation because of its high resolution; more than one thousand proteins or polypeptides can be separated according to the differences of their isoelectric point (pI) and molecular mass. However, 2−D PAGE involves many steps of manual handling, sample injection, setting gel molds in an electrophores...

Isoelectric focusing.

Utility of isoelectric focusing of cerebrospinal fluid and serum on agarose evaluated for neurological patients.

Agarose isoelectric focusing was used to demonstrate oligoclonal bands in cerebrospinal fluid (CSF) and serum from 998 consecutive neurological patients. Compared with agarose electrophoresis, agarose isoelectric focusing was slightly more sensitive, showing more (and more easily discernible) oligoclonal bands. Agarose isoelectric focusing, which has good reproducibility, revealed oligoclonal b...